Total RNA-Seq

Clontech

• Preferred method for analyzing both coding and non-coding RNA
• Generating libraries from inputs of varying quantity of RNA 
• For RNA of any integrity (RIN 2-10), including degraded RNA (e.g. FFPE samples)
• Kits use random hexamers for priming to generate transcriptomic data from an RNA sample
• Significant challenges is the typical abundance of ribosomal RNA in total RNA inputs
• Identification of non-coding and antisense RNA without strand-of-origin information
• Ligation-free protocols
• Illumina-ready sequencing libraries
• Multiplexing of up to 96 samples

 

1) Pico-input total RNA-seq (250 pg - 100 ng RNA)

SMARTer  Stranded Total RNA-Seq Kit v2– Pico Input Mammalian

• Inputs of 250 pg - 10 ng of mammalian total RNA
• RIN 2-10

 

2) Low-input total RNA-seq (100 pg - 100 ng RNA)

SMARTer Stranded RNA-Seq Kit

• Inputs of 10-100 ng of eukaryotic or prokaryotic RNA 
• RIN 3-10

SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian

• Inputs of 10-100 ng of total mammalian RNA 
• RIN 3-10

SMARTer  Universal Low Input RNA Kit for Sequencing

• Inputs of ≥200 pg of rRNA-depleted RNA of any species
• RIN 2-3 
• For Illumina or Ion Torrent

 

3) High-input total RNA-seq (100 ng - 1 µg RNA)

SMARTer  Stranded Total RNA Sample Prep Kit – HI Mammalian

• Inputs of 100 ng - 1 µg of mammalian total RNA
• RIN 3-10

 

 

Documents and resources

For more information visit producer's website.

SELECTION GUIDE Next-Gen Sequencing Kits