RetroNectin

TaKaRa Bio

RetroNectin Function

The RetroNectin Reagent is a recombinant human fibronectin fragment that contains three functional domains

• The cell-binding domain (C-domain)
• The heparin-binding domain (H-domain)
• The CS-1 sequence

 

RetroNectin reagent enhances lentiviral and retroviral transduction. Specifically, virus particles bind RetroNectin reagent via interaction with the H-domain, and target cells bind mainly through the interaction of cell surface integrin receptors VLA-5 and/or VLA-4 with the fibronectin C-domain and CS-1 sites.

 

 

1) Cell types ideal for RetroNectin

• Highly effective for cells that express integrin VLA-4 or VLA-5
• For T-cells, B-cells, monocytes, NK cells, eosinophils, bone marrow monocytic cells, thymocytes and lymphoid progenitors
• When using hematopoietic stem cells may be necessary to use cytokine pre-stimulation

 

2) Clinical GMP Grade RetroNectin

• It is possible to have RetroNectin GMP Grade for Clinical use or to have it directly without the need for a Material Transfer Agreement (MTA)
•  The Drug Master File for RetroNectin GMP grade has been filed with the U.S. Food and Drug Administration
• Clinical grade RetroNectin reagent has been used in over 68 protocols for gene therapy clinical trials at 44 institutions worldwide

 

 

Documents and resources

For more information visit producer's website.