Cybio FeliX High-Throughput Extraction

Analytik Jena

The Smart Extraction fundamentally alters the process of isolating and purifying nucleic acids is by eliminating the need for a number of work sequences, extra materials and specialized equipment. The Smart Modified Surface ensures that the entire process takes place right inside the pipette tip. Cybio FeliX  also provides high throughput automation of isolation of plasmid DNA from bacteria and bacterial transformation.

 

SmartExtraction CyBioFeliX

• For 1-96 samples
• Established kits
  • smart DNA prep(a) –extern lysistissuesamples, cells(eukaryotic, bacteria, yeast), rodenttails
  • smart Blood DNA Midi prep(a) –extern lysisblood < 3mL and buffycoat
  • smart Blood Midi Directprep(a) –intern lysisblood < 1mL and buffycoat
• Upcoming methods for all SmartExtraction kits
  • time saving; improving of DNA quality for downstream applications (NGS; droplet-PCR; etc.)

 

 

SmartExtraction CyBio FeliX–Lab ware configuration

• CyBio-FeliXBasic Unit with Enclosure, CyBio FeliX Head R 96/1000 μl
• 8-Channel Adapter; Head R 96, TipRack 96/1000 μl
• 96-Channel Magazin; Head R 96/1000 μl, WasteBox I
• PC System, CyBioComposer, Gripper, BioShake3000-T elm(QInstruments)
• Mounting Kit; BioShake3000 Series
• MicroplateAdapter for Axygen-plates(QInstruments)

 

Pipetting Heads

• For selective tip loading in single, row or column format (using cones)
• 1, 8, 12, 16, 24 channel liquid handling adapter available
• Automatic adapter change, identification and tip ejection within a method
• Automatic tip pick up and disposal by adapter from tip box

Isolation of plasmid DNA from bacterial cultures

• ​For 1-96 samples in approximately 1.5 hours
• Isolation of high quality plasmid DNA
• Yields suitable for downstream applications such as sequencing or transformation
• 96-well silica filter-plate plasmid extraction kit
• The compact platform reduces the bench space
• It can be  combinated with a bench top centrifuge and robotic arm

 

Suggested set-up positions for the CyBio FeliX deck. Each RoboTipTray is used for a specific reagents, according to its position.

 

Bacterial transformation

• For 1-96 samples
• Fully automated method for the high-throughput transformation and growth of bacteria cells
• Multi-well plate based method for transformation of DNA plasmid into bacteria cells by heat shock
• Using of additional heating/cooling blocks

CyBio FeliX deck layout for the automated transformation method

 

Documents and resources

For more information visit producer's website.

High-throughput plasmid DNA isolation from bacteria cells

High-throughput transformation of bacteria cells